| | クラブ | マネージャー | スタジアム | 本拠地 |
 | AV Alta FC | 未知の | The Hangar | Lancaster |
| Brooklyn FC | 未知の | Future Legends Complex | Brooklyn, New York |
| Central Valley Fuego | 未知の | Parmer Field | Fresno, California |
| Chattanooga Red Wolves | 未知の | David Stanton Field | Chattanooga |
| Detroit City | Danny Dichio | Keyworth Stadium | Detroit |
| FC Naples | 未知の | Paradise Coast Sports Complex Stadium | Naples |
| FC Tucson | 未知の | Kino Sports Complex | Tucson |
| Forward Madison | Matt Glaeser | Breese Stevens Field | Madison |
| Greenville Triumph SC | 未知の | Legacy Early College | Greenville |
| Inter Miami CF II | Cristian Ledesma | DRV PNK Stadium | Miami |
| Lexington SC | Terry Boss | Toyota Stadium | Lexington |
| New England Revolution II | 未知の | Gillette Stadium | Boston |
| North Carolina FC | John Bradford | WakeMed Soccer Park | Cary |
| North Texas SC | John Gall | Toyota Stadium | Frisco |
| Northern Colorado Hailstorm | 未知の | Future Legends Complex | Windsor, Colorado |
| One Knoxville SC | Ian Fuller | Highmark Stadium | Knoxville, Tennessee |
| Portland Hearts of Pine | 未知の | Fitzpatrick Stadium | Portland |
| Richmond Kickers | Darren Sawatzky | City Stadium | Richmond |
| San Diego FC | Mikey Varas | Snapdragon Stadium | San Diego |
| Santa Barbara Sky | 未知の | Titan Stadium | Santa Barbara, California |
| Spokane Velocity FC | 未知の | Skyline Sports Complex | Spokane, Washington |
| Texoma FC | Adrian Forbes | Torero Stadium | Sherman, Texas |
| Tormenta FC | Ian Cameron | Erk Russell Park | Statesboro |
| Toronto FC II | 未知の | Ontario Soccer Centre | Toronto |
| Union Omaha | 未知の | Werner Park | Omaha, Nebraska |
| Westchester SC | 未知の | The Stadium at Memorial Field | Westchester County |
